Human ELAV-like protein 1(ELAVL1) ELISA kit

Human ELAV-like protein 1(ELAVL1) ELISA kit

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Mfr.No.

CSB-EL007592HU

Alternative Names

ELAV (embryonic lethal, abnormal vision, Drosophila)-like 1 (Hu antigen R), ELAV1, HUR, Hua, MelG, ELAV-like 1, Hu antigen R, embryonic lethal, abnormal vision, drosophila, homolog-like 1

Reactivity

Human

Detection Range

25 pg/mL-1600 pg/mL

Sample Type

Cell lysates, Serum, Plasma, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human sialidase in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	85
	Range %	80-90
1:2	Average %	94
	Range %	91-98
1:4	Average %	98
	Range %	92-105
1:8	Average %	95
	Range %	86-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.621	2.522	2.572	2.460
10	2.212	2.112	2.162	2.050
5	1.701	1.654	1.678	1.566
2.5	1.257	1.197	1.227	1.115
1.25	0.763	0.741	0.752	0.640
0.625	0.461	0.434	0.448	0.336
0.312	0.287	0.273	0.280	0.168
0	0.115	0.109	0.112	?

Species

Homo sapiens

Sensitivity

6.25 pg/mL

Limit Of Detection

450 nm

UniProt ID	Q15717
Antigen	ELAV (embryonic lethal, abnormal vision, Drosophila)-like 1 (Hu antigen R)

Applications

ELISA

Research Area

ELAVL1

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10502216	CSB-EL007592HU	96T	$695	Add to Cart
BHE10502216	CSB-EL007592HU	5x96T	$3336	Add to Cart
BHE10502216	CSB-EL007592HU	10x96T	$6602.50	Add to Cart

The recovery of human sialidase spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type

Average % Recovery

Serum (n=5)