Human elastase 2, neutrophil (ELA2)ELISA kit

Mfr.No.

CSB-EL007587HU

Sensitivity

1.56 ng/mL

Detection Range

6.25 ng/mL-400 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

elastase 2, neutrophil (ELA2), ELA2, GE, HLE, HNE, NE, PMN-E, SCN1, bone marrow serine protease, elastase 2, neutrophil, granulocyte-derived elastase, leukocyte elastase, medullasin, neutrophil elastase, polymorphonuclear elastase

Recovery

The recovery of human BDKRB1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-98
EDTA plasma (n=4)	92	87-96

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human BDKRB1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	102
	Range %	99-107
1:2	Average %	97
	Range %	91-103
1:4	Average %	92
	Range %	87-98
1:8	Average %	104
	Range %	96-108

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1600	1.774	1.845	1.810	1.624
800	1.321	1.428	1.375	1.189
400	1.015	0.982	0.999	0.813
200	0.712	0.701	0.707	0.521
100	0.453	0.469	0.461	0.275
50	0.311	0.324	0.318	0.132
25	0.242	0.249	0.246	0.060
0	0.185	0.187	0.186	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	P08246

Applications	ELISA
Research Area	ELA2

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10502214	CSB-EL007587HU	96T	$790	Add to Cart
BHE10502214	CSB-EL007587HU	5x96T	$3792	Add to Cart
BHE10502214	CSB-EL007587HU	10x96T	$7505	Add to Cart

Human elastase 2, neutrophil (ELA2)ELISA kit

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