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Home / ELISA Kit

Human Elafin (PI3) ELISA Kit

SKU : BHE13703434

Elafin is an elastase-specific inhibitor, which contains a WAP-type four-disulfide core (WFDC) domain, and is thus a member of the WFDC domain family. Chang et al. and Schalkwijk et al. described a low molecular weight elastase inhibitor derived from psoriatic skin and termed SKALP for 'skin-derived antileukoproteinase.' They demonstrated that this inhibitor can be found in the epidermis of several inflammatory skin diseases, but not in normal human epidermis. Wiedow et al. referred to this molecule as elafin, and Sallenave and Ryle referred to it as elastase-specific inhibitor. It is found in the urine of psoriatic patients, and immunohistochemical studies showed that SKALP/elafin is found in the suprabasal differentiated keratinocytes of psoriatic epidermis. Molhuizen et al. isolated a precursor of SKALP/elafin from cultured keratinocytes and cloned its cDNA.

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Mfr.No.	KTE61218
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Elafin (PI3) ELISA Kit employs a two-site sandwich ELISA to quantitate PI3.
Alternative Names	PI3; ESI; MGC13613; SKALP; WAP3; WFDC14; cementoin; WAP four-disulfide core domain 14; elafin; elastase-specific inhibitor; pre-elafin; protease inhibitor 3; skin-derived (SKALP); skin-derived antileukoprotei
Detection Method	Colorimetric
Kit Components	Human Elafin microplate Human Elafin standard Human Elafin detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Elafin (PI3) ELISA Kit has high sensitivity and excellent specificity for detection of Human PI3. No significant cross-reactivity or interference between Human PI3 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Elafin is an elastase-specific inhibitor, which contains a WAP-type four-disulfide core (WFDC) domain, and is thus a member of the WFDC domain family. Chang et al. and Schalkwijk et al. described a low molecular weight elastase inhibitor derived from psoriatic skin and termed SKALP for 'skin-derived antileukoproteinase.' They demonstrated that this inhibitor can be found in the epidermis of several inflammatory skin diseases, but not in normal human epidermis. Wiedow et al. referred to this molecule as elafin, and Sallenave and Ryle referred to it as elastase-specific inhibitor. It is found in the urine of psoriatic patients, and immunohistochemical studies showed that SKALP/elafin is found in the suprabasal differentiated keratinocytes of psoriatic epidermis. Molhuizen et al. isolated a precursor of SKALP/elafin from cultured keratinocytes and cloned its cDNA.
Notes	This Human Elafin (PI3) ELISA Kit employs a two-site sandwich ELISA to quantitate PI3 in samples. An antibody specific for PI3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPI3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PI3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PI3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	5266

Applications	ELISA
Applications Notes	This Human Elafin (PI3) ELISA Kit employs a two-site sandwich ELISA to quantitate PI3 in samples. An antibody specific for PI3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPI3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PI3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PI3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703434	KTE61218	48 T	$339		Add to Cart
BHE13703434	KTE61218	96 T	$589		Add to Cart
BHE13703434	KTE61218	96 Tx5	$2629		Add to Cart
BHE13703434	KTE61218	96 Tx50	$24569		Add to Cart

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