Human Elafin - P13 ELISA Kit

Mfr.No.

E4762Hu

Sensitivity

3.05ng/L

Detection Range

5-1000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Elafin (also known as P13) in serum, plasma, cell culture supeates, cell lysates, tissue homogenates.

Alternative Names

Elafin;Elastase-specific inhibitor;ESI;Peptidase inhibitor 3;PI 3;PI3;PI-3;Protease inhibitor WAP3;SKALP;Skin-derived antileukoproteinase;WAP four-disulfide core domain protein 14

Molecular Weight

12270

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supeatants, Plasma

Precision

We measured random samples of E4762Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	206.2	7.93	3.8
Intra-Assay	2	18	365.5	8.04	2.2
Intra-Assay	3	18	99.7	4.93	4.9

Background

Neutrophil and pancreatic elastase-specific inhibitor of skin. It may prevent elastase-mediated tissue proteolysis. Has been shown to inhibit the alpha-4-beta-2/CHRNA2-CHRNB2 nicotinic acetylcholine receptor and to produce a weak inhibition on Kv11.1/KCNH2/ERG1 and on the transient receptor potential cation Channel subfamily V member 1 (TRPV1) (PubMed: 29483648).

Protocol

These standard curves of E4762Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
640ng/L	2.433	2.409	2.378
	2.385
320ng/L	1.26	1.274	1.243
	1.288
160ng/L	0.602	0.58	0.549
	0.559
80ng/L	0.335	0.346	0.315
	0.357
40ng/L	0.194	0.184	0.153
	0.173
0ng/L	0.034	0.031	0
	0.028

Gene ID	5266
UniProt ID	P19957

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106049	E4762Hu	48T	$320		Add to Cart
BHE12106049	E4762Hu	96T	$458		Add to Cart

Human Elafin - P13 ELISA Kit

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