Human EGF ELISA Kit EZ-Set^TM (DIY Antibody Pairs)

Mfr.No.

EZ0325

Detection Range

4.7 pg/ml - 300 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	4.7	9.4	18.8	37.5	75	150	300
O.D.	0.046	0.092	0.151	0.265	0.521	0.943	1.504	1.965

Description

Human EGF ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human EGF in cell culture supeatants, serum, plasma (heparin, EDTA, citrate) and urine.

Alternative Names

Pro-epidermal growth factor;EGF;Epidermal growth factor;Urogastrone;EGF;

Molecular Weight

133994 MW

Immunogen

Expression system for standard: E.coli; Immunogen sequence: N971-R1023

Kit Components

Description
Capture Antibody
Detection Antibody
Avidin-Biotin-Peroxidase Complex (ABC)
Lyophilized Standard

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human EGF

Subcellular Localization

Membrane; Single-pass type I membrane protein.

Sample Type

Cell culture supeatants, Serum, Plasma, Urine

Anticoagulant

heparin, EDTA or citrate

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	42.1	105	197	45.2	112	227
Standard deviation	1.98	5.67	6.3	3.21	5.71	13.17
CV (%)	4.7%	5.4%	3.2%	7.1%	5.1%	5.8%

Protein Function

EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941). .

Background

Epidermal growth factor or EGF is a growth factor that plays an important role in the regulation of cell growth, proliferation, and differentiation by binding to its receptor EGFR. Human EGF is a 6045-Da protein with 53 amino acid residues and three intramolecular disulfide bonds. EGF acts by binding with high affinity to epidermal growth factor receptor (EGFR) on the cell surface and stimulating the intrinsic protein-tyrosine kinase activity of the receptor. EGF results in cellular proliferation, differentiation, and survival. It also has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. EGF has strong expression in kidney, salivary gland, cerebrum, and prostate, moderate expression in trachea and thyroid, and low expression in bone marrow, heart, spleen, thymus, uterus, and colon. No expression was detected in adrenal gland, liver, lung, cerebellum, placenta, and small intestine.

Notes

Material Required But Not Provided:
1. Microplate reader in standard size.
2. Automated plate washer.
3. Incubator.
4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
5. Clean tubes and Eppendorf tubes.
6. 96 well microplate
7. Plate Sealers.
8. Capture Antibody Diluent: PBS.
9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
10. Substrate Solution: Tetramethylbenzidine
11. Stop Solution: 2 N H2SO4
12. Wash Buffer . Recipes:
PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
*Item 6 12 are included in the EZ Set Accessory Kit

Gene ID	1950
Gene Name	EGF
UniProt ID	E7EVD2
Protein Name	Pro-epidermal growth factor
Tissue Specificity	Expressed in kidney, salivary gland, cerebrum and prostate. .
Sequence	Contains 9 EGF-like domains.

Applications	ELISA
Research Area	Cancer,Cell Biology,Cell Cycle,Cell Differentiation,Growth Factors,Growth Factors/Hormones,Signal Transduction

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000004	EZ0325	5 plates/kit	$500		Add to Cart

Human EGF ELISA Kit EZ-Set^TM (DIY Antibody Pairs)

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