Human EGF-containing Fibulin-like Extracellular Matrix Protein 1

Mfr.No.

E5228Hu

Alternative Names

DHRD;DRAD;EFEMP1;EGF-containing fibulin-like extracellular matrix protein 1;Extracellular protein S1-5;FBNL;FIBL-3;Fibrillin-like protein;Fibulin-3;MLVT;MTLV;S1-5

Reactivity

Human

Detection Range

0.5-200ng/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

54641

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.33ng/ml

Background

Binds EGFR, the EGF receptor, inducing EGFR autophosphorylation and the activation of downstream signaling pathways. May play a role in cell adhesion and migration. May function as a negative regulator of chondrocyte differentiation. In the olfactory epithelium, it may regulate glial cell migration, differentiation and the ability of glial cells to support neuronal neurite outgrowth.

Gene ID	2202
UniProt ID	Q12805
Antigen	EFEMP1

Research Area

Signal Transduction

Protocol

These standard curves of E5228Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
120ng/ml	1.959	1.98	1.932
	2.002
60ng/ml	1.409	1.459	1.41
	1.509
30ng/ml	0.684	0.643	0.595
	0.603
15ng/ml	0.432	0.439	0.391
	0.446
7.5ng/ml	0.196	0.18	0.131
	0.163
0ng/ml	0.046	0.048	0
	0.051

Precision

We measured random samples of E5228Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	45.76	1.91	4.18
Intra-Assay	2	18	84.59	4.48	5.3
Intra-Assay	3	18	19.95	0.63	3.16

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106591	E5228Hu	48T	$320		Add to Cart
BHE12106591	E5228Hu	96T	$458		Add to Cart

Human EGF-containing Fibulin-like Extracellular Matrix Protein 1 - EFEMP1 ELISA Kit

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