Matrices listed below were spiked with certain level of EFCAB14 and the recovery rates were calculated by comparing the measured value to the expected amount of EFCAB14 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-101
96
EDTA plasma(n=5)
85-98
90
heparin plasma(n=5)
86-105
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of EFCAB14 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
94-100%
86-104%
86-104%
EDTA plasma(n=5)
87-98%
84-101%
88-97%
heparin plasma(n=5)
85-100%
81-99%
89-99%
Sensitivity
< 46.875pg/ml
Notes
For Research Use Only
UniProt ID
O75071
Applications
ELISA
Applications Notes
EFCAB14 ELISA Kit allows for the in vitro quantitative determination of EFCAB14 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human EFCAB14 (EF-hand calcium-binding domain-containing protein 14) ELISA Kit