Matrices listed below were spiked with certain level of EEF2K and the recovery rates were calculated by comparing the measured value to the expected amount of EEF2K in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
95-101
98
EDTA plasma(n=5)
95-105
98
heparin plasma(n=5)
95-102
98
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of EEF2K and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
90-103%
89-102%
93-105%
EDTA plasma(n=5)
92-98%
90-98%
91-98%
heparin plasma(n=5)
91-98%
90-100%
92-99%
Sensitivity
< 9.375pg/ml
Notes
For Research Use Only
UniProt ID
O00418
Applications
ELISA
Applications Notes
EEF2K ELISA Kit allows for the in vitro quantitative determination of EEF2K concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human EEF2K (Eukaryotic elongation factor 2 kinase) ELISA Kit