Human Ectonucleotide pyrophosphatase/phosphodiesterase family member 2(ENPP2) ELISA kit

Human Ectonucleotide pyrophosphatase/phosphodiesterase family member 2(ENPP2) ELISA kit

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Mfr.No.

CSB-EL007680HU

Alternative Names

ectonucleotide pyrophosphatase/phosphodiesterase 2, ATX, ATX-X, AUTOTAXIN, FLJ26803, LysoPLD, NPP2, PD-IALPHA, PDNP2, autotaxin, autotaxin-t, phosphodiesterase I/nucleotide pyrophosphatase 2, plasma lysophospholipase D

Reactivity

Human

Detection Range

25 ng/mL-2000 ng/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Competitive

Assay Duration

1-5h

Recovery

The recovery of human TNFRSF25 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	83	80-86
EDTA plasma (n=4)	91	88-95

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human TNFRSF25 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	85-93
1:2	Average %	94
	Range %	91-99
1:4	Average %	84
	Range %	81-89
1:8	Average %	90
	Range %	87-93

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
15	2.464	2.498	2.481	2.321
7.5	1.696	1.668	1.682	1.522
3.75	1.101	1.135	1.118	0.958
1.88	0.712	0.732	0.722	0.562
0.94	0.513	0.536	0.525	0.365
0.47	0.398	0.387	0.393	0.233
0.24	0.297	0.276	0.287	0.127
0	0.161	0.159	0.160

Species

Homo sapiens

Sensitivity

31.25 ng/mL

Limit Of Detection

450 nm

UniProt ID	Q13822
Antigen	Ectonucleotide Pyrophosphatase/Phosphodiesterase 2

Applications

ELISA

Research Area

ENPP2

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10502204	CSB-EL007680HU	96T	$595	Add to Cart
BHE10502204	CSB-EL007680HU	5x96T	$2856	Add to Cart
BHE10502204	CSB-EL007680HU	10x96T	$5652.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.