Human Ectonucleotide pyrophosphatase/phosphodiesterase family member 2(ENPP2) ELISA kit

Human Ectonucleotide pyrophosphatase/phosphodiesterase family member 2(ENPP2) ELISA kit

Reviews of Human Ectonucleotide pyrophosphatase/phosphodiesterase family member 2(ENPP2) ELISA kit

Rate it now..!!

Rate it now..!!

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Mfr.No.

CSB-EL007680HU

Sensitivity

31.25 ng/mL

Detection Range

25 ng/mL-2000 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Competitive

Alternative Names

ectonucleotide pyrophosphatase/phosphodiesterase 2, ATX, ATX-X, AUTOTAXIN, FLJ26803, LysoPLD, NPP2, PD-IALPHA, PDNP2, autotaxin, autotaxin-t, phosphodiesterase I/nucleotide pyrophosphatase 2, plasma lysophospholipase D

Recovery

The recovery of human TNFRSF25 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	83	80-86
EDTA plasma (n=4)	91	88-95

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human TNFRSF25 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	85-93
1:2	Average %	94
	Range %	91-99
1:4	Average %	84
	Range %	81-89
1:8	Average %	90
	Range %	87-93

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
15	2.464	2.498	2.481	2.321
7.5	1.696	1.668	1.682	1.522
3.75	1.101	1.135	1.118	0.958
1.88	0.712	0.732	0.722	0.562
0.94	0.513	0.536	0.525	0.365
0.47	0.398	0.387	0.393	0.233
0.24	0.297	0.276	0.287	0.127
0	0.161	0.159	0.160

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q13822

Applications	ELISA
Research Area	ENPP2

Storage	Store at 2-8°C. Please refer to protocol.

Have you used this product?

SKU	Supplier No.	Size	Your price
BHE10502204	CSB-EL007680HU	96T	$595	Add to Cart
BHE10502204	CSB-EL007680HU	5x96T	$2856	Add to Cart
BHE10502204	CSB-EL007680HU	10x96T	$5652.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.