Human Ectonucleoside Triphosphate Diphosphohydrolase 1

Mfr.No.

E5222Hu

Sensitivity

8.04ng/L

Detection Range

15-3000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

ATPDase;CD antigen CD39;CD39;Ecto-apyrase;Ecto-ATP diphosphohydrolase 1;Ecto-ATPase 1;Ecto-ATPDase 1;Ectonucleoside triphosphate diphosphohydrolase 1;ENTPD1;Lymphoid cell activation antigen;NTPDase 1;NTPDase-1;SPG64;

Molecular Weight

57965

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E5222Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	374.88	19.02	5.07
Intra-Assay	2	18	1156.3	43.22	3.74
Intra-Assay	3	18	204	12.48	6.12

Background

In the nervous system, could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. Could also be implicated in the prevention of platelet aggregation by hydrolyzing platelet-activating ADP to AMP. Hydrolyzes ATP and ADP equally well.

Protocol

These standard curves of E5222Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	2.115	2.116	2.062
	2.116
800ng/L	1.378	1.449	1.395
	1.519
400ng/L	0.821	0.833	0.779
	0.844
200ng/L	0.423	0.463	0.409
	0.503
100ng/L	0.314	0.297	0.243
	0.28
0ng/L	0.056	0.054	0
	0.051

Gene ID	953
UniProt ID	P49961

Research Area	Immunology

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106585	E5222Hu	48T	$320		Add to Cart
BHE12106585	E5222Hu	96T	$458		Add to Cart

Human Ectonucleoside Triphosphate Diphosphohydrolase 1 - ENTPD1 ELISA Kit

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