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Home / ELISA Kit

Human early prostate cancer antigen 2 (EPCA2) ELISA kit

SKU : BHE10502195

This Human GPC3 ELISA Kit was designed for the quantitative measurement of Human GPC3 protein in serum, urine, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL.

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CUSABIO TECHONOLOGY LLC

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Mfr.No.

CSB-EQ027679HU

Sensitivity

6.25 ng/mL

Detection Range

12.5 ng/mL-400 ng/mL

Reactivity

Human

Assay Duration

1-5h

Alternative Names

early prostate cancer antigen 2 (EPCA2)

Recovery

The recovery of human GPC-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type		Average % Recovery		Range
Serum (n=5)		95		89-105

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human GPC-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?		Sample		Serum(n=4)
1:1		Average %		90
		Range %		80-100
1:2		Average %		98
		Range %		91-105
1:4		Average %		100
		Range %		92-110
1:8		Average %		93
		Range %		86-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
40	2.621	2.641	2.631	2.437
20	2.356	2.194	2.275	2.081
10	1.626	1.674	1.650	1.456
5	1.021	1.101	1.061	0.867
2.5	0.596	0.600	0.598	0.404
1.25	0.412	0.422	0.417	0.223
0.625	0.302	0.315	0.309	0.115
0	0.187	0.201	0.194	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

Protocol

Applications	ELISA
Research Area	EPCA2

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price	Quantity
BHE10502195	CSB-EQ027679HU	96T	$695		Add to Cart
BHE10502195	CSB-EQ027679HU	5x96T	$3336		Add to Cart
BHE10502195	CSB-EQ027679HU	10x96T	$6602.50		Add to Cart

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