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Home / ELISA Kit

Human E3 ubiquitin-protein ligase UBR4 (UBR4) ELISA Kit

SKU : BHE13702313

RBAF600 associates with RB in the nucleus and with Ca(2+)-bound calmodulin in the cytoplasm.p600 and RB appears to act as a chromatin scaffold, and in the cytoplasm p600 and clathrin form a meshwork structure which could contribute to cytoskeletal organization and membrane morphogenesis. Reduced expression of p600 with interfering RNA abrogated integrin-mediated ruffled membrane formation and prevented activation of integrin-mediated survival pathways. The deduced 5,183-amino acid protein has a calculated molecular mass of 573.5 kD. Immunostaining of human foreskin fibroblasts localized p600 in a continuous meshwork pattern from the nucleus to the cytoplasm, passing through the nuclear envelope. In the cytoplasm, p600 was concentrated at the leading edge of membrane structures.

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Mfr.No.	KTE60094
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human E3 ubiquitin-protein ligase UBR4 (UBR4) ELISA Kit employs a two-site sandwich ELISA to quantitate UBR4.
Alternative Names	UBR4; RP5-1126H10.1; FLJ41863; KIAA0462; KIAA1307; RBAF600; ZUBR1; p600; retinoblastoma-associated factor 600; retinoblastoma-associated factor 600-like protein; zinc finger; UBR1 type 1
Detection Method	Colorimetric
Kit Components	Human E3 ubiquitin-protein ligase UBR4 microplate Human E3 ubiquitin-protein ligase UBR4 standard Human E3 ubiquitin-protein ligase UBR4 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human E3 ubiquitin-protein ligase UBR4 (UBR4) ELISA Kit has high sensitivity and excellent specificity for detection of Human UBR4. No significant cross-reactivity or interference between Human UBR4 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	RBAF600 associates with RB in the nucleus and with Ca(2+)-bound calmodulin in the cytoplasm.p600 and RB appears to act as a chromatin scaffold, and in the cytoplasm p600 and clathrin form a meshwork structure which could contribute to cytoskeletal organization and membrane morphogenesis. Reduced expression of p600 with interfering RNA abrogated integrin-mediated ruffled membrane formation and prevented activation of integrin-mediated survival pathways. The deduced 5,183-amino acid protein has a calculated molecular mass of 573.5 kD. Immunostaining of human foreskin fibroblasts localized p600 in a continuous meshwork pattern from the nucleus to the cytoplasm, passing through the nuclear envelope. In the cytoplasm, p600 was concentrated at the leading edge of membrane structures.
Notes	This Human E3 ubiquitin-protein ligase UBR4 (UBR4) ELISA Kit employs a two-site sandwich ELISA to quantitate UBR4 in samples. An antibody specific for UBR4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyUBR4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UBR4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UBR4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	23352

Applications	ELISA
Applications Notes	This Human E3 ubiquitin-protein ligase UBR4 (UBR4) ELISA Kit employs a two-site sandwich ELISA to quantitate UBR4 in samples. An antibody specific for UBR4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyUBR4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UBR4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UBR4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702313	KTE60094	48 T	$339		Add to Cart
BHE13702313	KTE60094	96 T	$589		Add to Cart
BHE13702313	KTE60094	96 Tx5	$2629		Add to Cart
BHE13702313	KTE60094	96 Tx50	$24569		Add to Cart

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