Human E3 Ubiquitin-protein Ligase Trim68

Mfr.No.

E5219Hu

Sensitivity

0.015ng/ml

Detection Range

0.05-20ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

E3 ubiquitin-protein ligase TRIM68;GC109;RING finger protein 137;RING-type E3 ubiquitin transferase TRIM68;RNF137;SS56;SS-56;SSA protein SS-56;TRIM68;Tripartite motif-containing protein 68

Molecular Weight

56259

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E5219Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	4.97	0.17	3.49
Intra-Assay	2	18	4.45	0.27	5.99
Intra-Assay	3	18	2.17	0.12	5.63

Background

Functions as a ubiquitin E3 ligase. Acts as a coactivator of androgen receptor (AR) depending on its ubiquitin ligase activity.

Protocol

These standard curves of E5219Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
12ng/ml	2.451	2.398	2.349
	2.346
6ng/ml	1.583	1.557	1.508
	1.531
3ng/ml	0.881	0.856	0.807
	0.83
1.5ng/ml	0.46	0.494	0.445
	0.529
0.75ng/ml	0.262	0.266	0.217
	0.27
0ng/ml	0.055	0.049	0
	0.042

Gene ID	55128
UniProt ID	Q6AZZ1

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106582	E5219Hu	48T	$320		Add to Cart
BHE12106582	E5219Hu	96T	$458		Add to Cart

Human E3 Ubiquitin-protein Ligase Trim68 - TRIM68 ELISA Kit

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