Human E3 Ubiquitin-protein Ligase Trim68

Mfr.No.

E5219Hu

Alternative Names

E3 ubiquitin-protein ligase TRIM68;GC109;RING finger protein 137;RING-type E3 ubiquitin transferase TRIM68;RNF137;SS56;SS-56;SSA protein SS-56;TRIM68;Tripartite motif-containing protein 68

Reactivity

Human

Detection Range

0.05-20ng/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

56259

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.015ng/ml

Background

Functions as a ubiquitin E3 ligase. Acts as a coactivator of androgen receptor (AR) depending on its ubiquitin ligase activity.

Gene ID	55128
UniProt ID	Q6AZZ1
Antigen	TRIM68

Research Area

Others

Protocol

These standard curves of E5219Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
12ng/ml	2.451	2.398	2.349
	2.346
6ng/ml	1.583	1.557	1.508
	1.531
3ng/ml	0.881	0.856	0.807
	0.83
1.5ng/ml	0.46	0.494	0.445
	0.529
0.75ng/ml	0.262	0.266	0.217
	0.27
0ng/ml	0.055	0.049	0
	0.042

Precision

We measured random samples of E5219Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	4.97	0.17	3.49
Intra-Assay	2	18	4.45	0.27	5.99
Intra-Assay	3	18	2.17	0.12	5.63

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106582	E5219Hu	48T	$320		Add to Cart
BHE12106582	E5219Hu	96T	$458		Add to Cart

Human E3 Ubiquitin-protein Ligase Trim68 - TRIM68 ELISA Kit

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