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Home / ELISA Kit

Human E3 Ubiquitin-protein Ligase Synoviolin - SYVN1 ELISA Kit

SKU : BHE12106580

E3 ubiquitin-protein ligase synoviolin is an enzyme that in humans is encoded by the SYVN1 gene.

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Bioassay Technology Laboratory

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Sample Request

Mfr.No.

E5217Hu

Sensitivity

2.99ng/L

Detection Range

5-1000ng/L

Reactivity

Human

Sample Type

Serum, Cell culture supernatants, Plasma

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

DER3;E3 ubiquitin-protein ligase synoviolin;HRD1;RING-type E3 ubiquitin transferase synoviolin;Synovial apoptosis inhibitor 1;SYVN1

Molecular Weight

67685

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E5217Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	169.56	10.92	6.44
Intra-Assay	2	18	227.64	10.27	4.51
Intra-Assay	3	18	42.93	3.39	7.91

Background

E3 ubiquitin-protein ligase synoviolin is an enzyme that in humans is encoded by the SYVN1 gene.

Protocol

These standard curves of E5217Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
640ng/L	2.385	2.375	2.326
	2.366
320ng/L	1.624	1.603	1.553
	1.582
160ng/L	1.14	1.057	1.007
	0.974
80ng/L	0.628	0.629	0.579
	0.63
40ng/L	0.445	0.437	0.388
	0.43
0ng/L	0.051	0.05	0
	0.049

Gene ID	84447
UniProt ID	Q86TM6
Antigen	SYVN1

Research Area	Cell Biology

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106580	E5217Hu	48T	$320		Add to Cart
BHE12106580	E5217Hu	96T	$458		Add to Cart

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