Human E3 Ubiquitin-protein Ligase Smurf1

Mfr.No.

E6630Hu

Alternative Names

E3 ubiquitin-protein ligase SMURF1;HECT-type E3 ubiquitin transferase SMURF1;hSMURF1;SMAD ubiquitination regulatory factor 1;SMAD-specific E3 ubiquitin-protein ligase 1;SMURF1

Reactivity

Human

Detection Range

20-4500ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

86114

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

12.28ng/L

Background

E3 ubiquitin-protein ligase that acts as a negative regulator of BMP signaling pathway. Mediates ubiquitination and degradation of SMAD1 and SMAD5, 2 receptor-regulated SMADs specific for the BMP pathway. Promotes ubiquitination and subsequent proteasomal degradation of TRAF family members and RHOA. Promotes ubiquitination and subsequent proteasomal degradation of MAVS (PubMed:23087404). Plays a role in dendrite formation by melanocytes (PubMed:23999003).

Gene ID	57154
UniProt ID	Q9HCE7
Antigen	SMURF1

Research Area

Cell Biology

Protocol

These standard curves of E6630Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	1.828	1.909	1.875
	1.99
1200ng/L	1.129	1.058	1.023
	0.986
600ng/L	0.54	0.474	0.439
	0.408
300ng/L	0.282	0.259	0.224
	0.235
150ng/L	0.129	0.104	0.07
	0.08
0ng/L	0.031	0.034	0
	0.038

Precision

We measured random samples of E6630Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1558.35	43.03	2.76
Intra-Assay	2	18	982.54	40.01	4.07
Intra-Assay	3	18	475.95	21.11	4.44

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12115322	E6630Hu	48T	$320		Add to Cart
BHE12115322	E6630Hu	96T	$458		Add to Cart

Human E3 Ubiquitin-protein Ligase Smurf1 - SMURF1 ELISA Kit

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