Human E3 Ubiquitin-protein Ligase Rad18

Mfr.No.

E5216Hu

Alternative Names

E3 ubiquitin-protein ligase RAD18;hHR18;hRAD18;Postreplication repair protein RAD18;RAD18;RING finger protein 73;RING-type E3 ubiquitin transferase RAD18;RNF73

Reactivity

Human

Detection Range

10-2000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

56223

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

5.01ng/L

Background

E3 ubiquitin-protein ligase RAD18 is an enzyme that in humans is encoded by the RAD18 gene.

Gene ID	56852
UniProt ID	Q9NS91
Antigen	RAD18

Research Area

Epigenetics and Nuclear Signaling

Protocol

These standard curves of E5216Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200ng/L	2.238	2.183	2.129
	2.128
600ng/L	1.494	1.594	1.539
	1.693
300ng/L	0.968	0.985	0.931
	1.003
150ng/L	0.736	0.759	0.705
	0.783
75ng/L	0.483	0.477	0.422
	0.47
0ng/L	0.051	0.055	0
	0.058

Precision

We measured random samples of E5216Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	128.68	11.15	8.67
Intra-Assay	2	18	234.72	18.29	7.79
Intra-Assay	3	18	579.34	27.76	4.79

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106579	E5216Hu	48T	$320		Add to Cart
BHE12106579	E5216Hu	96T	$458		Add to Cart

Human E3 Ubiquitin-protein Ligase Rad18 - RAD18 ELISA Kit

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