Human E3 Ubiquitin-protein Ligase Rad18

Mfr.No.

E5216Hu

Sensitivity

5.01ng/L

Detection Range

10-2000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

E3 ubiquitin-protein ligase RAD18;hHR18;hRAD18;Postreplication repair protein RAD18;RAD18;RING finger protein 73;RING-type E3 ubiquitin transferase RAD18;RNF73

Molecular Weight

56223

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E5216Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	128.68	11.15	8.67
Intra-Assay	2	18	234.72	18.29	7.79
Intra-Assay	3	18	579.34	27.76	4.79

Background

E3 ubiquitin-protein ligase RAD18 is an enzyme that in humans is encoded by the RAD18 gene.

Protocol

These standard curves of E5216Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200ng/L	2.238	2.183	2.129
	2.128
600ng/L	1.494	1.594	1.539
	1.693
300ng/L	0.968	0.985	0.931
	1.003
150ng/L	0.736	0.759	0.705
	0.783
75ng/L	0.483	0.477	0.422
	0.47
0ng/L	0.051	0.055	0
	0.058

Gene ID	56852
UniProt ID	Q9NS91

Research Area	Epigenetics and Nuclear Signaling

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106579	E5216Hu	48T	$320		Add to Cart
BHE12106579	E5216Hu	96T	$458		Add to Cart

Human E3 Ubiquitin-protein Ligase Rad18 - RAD18 ELISA Kit

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