Human E3 Ubiquitin-protein Ligase Mylip

Mfr.No.

E5215Hu

Sensitivity

5.24ng/L

Detection Range

10-2000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

E3 ubiquitin-protein ligase MYLIP;IDOL;Inducible degrader of the LDL-receptor;MIR;MYLIP;Myosin regulatory light chain interacting protein;RING-type E3 ubiquitin transferase MYLIP

Molecular Weight

49910

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E5215Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	514.75	17.63	3.43
Intra-Assay	2	18	448.54	26.34	5.87
Intra-Assay	3	18	144.38	7.05	4.88

Background

Myosin regulatory light chain interacting protein, also known as MYLIP, is a protein that in humans is encoded by the MYLIP gene. MYLIP is also known as IDOL "Inducible Degrader of the LDL receptor" based on its involvement in cholesterol regulation or MIR "Modulator Of Immune Recognition". The expression of IDOL is induced by the sterol-activated liver X receptor.

Protocol

These standard curves of E5215Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200ng/L	2.546	2.467	2.407
	2.389
600ng/L	1.495	1.445	1.385
	1.395
300ng/L	0.845	0.782	0.722
	0.718
150ng/L	0.448	0.488	0.428
	0.528
75ng/L	0.205	0.205	0.145
	0.205
0ng/L	0.065	0.06	0
	0.055

Gene ID	29116
UniProt ID	Q8WY64

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106578	E5215Hu	48T	$320		Add to Cart
BHE12106578	E5215Hu	96T	$458		Add to Cart

Human E3 Ubiquitin-protein Ligase Mylip - MYLIP ELISA Kit

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