Human E3 ubiquitin-protein ligase MYLIP(MYLIP) ELISA kit

Human E3 ubiquitin-protein ligase MYLIP(MYLIP) ELISA kit

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Mfr.No.

CSB-EL015319HU

Alternative Names

myosin regulatory light chain interacting protein, RP1-13D10.1, IDOL, MIR, band 4.1 superfamily member BZF1, cellular modulator of immune recognition (c-MIR)

Reactivity

Human

Detection Range

15.6 pg/mL-1000 pg/mL

Sample Type

Cell lysates, Serum, Plasma, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of rat IP3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	91-105
EDTA plasma (n=4)	95	92-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat IP3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	90
	Range %	85-96
1:2	Average %	94
	Range %	89-98
1:4	Average %	96
	Range %	87-102
1:8	Average %	97
	Range %	91-104

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	?
1000	0.141	0.143	0.142	?
250	0.270	0.261	0.266	?
80	0.423	0.440	0.432	?
20	0.744	0.759	0.752	?
5	1.156	1.162	1.159	?
0	2.199	2.214	2.207	?

Species

Homo sapiens

Sensitivity

3.9 pg/mL

Limit Of Detection

450 nm

UniProt ID	Q8WY64
Antigen	myosin regulatory light chain interacting protein

Applications

ELISA

Research Area

MYLIP

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10502186	CSB-EL015319HU	96T	$695	Add to Cart
BHE10502186	CSB-EL015319HU	5x96T	$3336	Add to Cart
BHE10502186	CSB-EL015319HU	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.