Human E3 Ubiquitin-protein Ligase Mdm2

Mfr.No.

E5213Hu

Sensitivity

9.33ng/L

Detection Range

20-4500ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

ACTFS;Double minute 2 protein;E3 ubiquitin-protein ligase Mdm2;hdm2;HDMX;LSKB;MDM2;Oncoprotein Mdm2;p53-binding protein Mdm2;RING-type E3 ubiquitin transferase Mdm2

Molecular Weight

55233

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E5213Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1138.77	44.72	3.93
Intra-Assay	2	18	354.45	24.31	6.86
Intra-Assay	3	18	992.83	60.69	6.11

Background

Mouse double minute 2 homolog (MDM2) also known as E3 ubiquitin-protein ligase Mdm2 is a protein that in humans is encoded by the MDM2 gene. Mdm2 is an important negative regulator of the p53 tumor suppressor. Mdm2 protein functions both as an E3 ubiquitin ligase that recognizes the N-terminal trans-activation domain (TAD) of the p53 tumor suppressor and as an inhibitor of p53 transcriptional activation.

Protocol

These standard curves of E5213Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	2.098	2.202	2.149
	2.307
1200ng/L	1.402	1.411	1.359
	1.42
600ng/L	0.863	0.895	0.842
	0.927
300ng/L	0.444	0.491	0.438
	0.538
150ng/L	0.221	0.245	0.192
	0.268
0ng/L	0.037	0.053	0
	0.068

Gene ID	4193
UniProt ID	Q00987

Research Area	Cell Biology

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106576	E5213Hu	48T	$320		Add to Cart
BHE12106576	E5213Hu	96T	$458		Add to Cart

Human E3 Ubiquitin-protein Ligase Mdm2 - MDM2 ELISA Kit

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