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Home / ELISA Kit

Human E3 ubiquitin-protein ligase CHIP (STUB1) ELISA Kit

SKU : BHE13702614

The CHIP protein encoded by this gene binds to and inhibits the ATPase activity of the chaperone proteins HSC70 and HSP70 and blocks the forward reaction of the HSC70-HSP70 substrate-binding cycle. In addition, CHIP possesses E3 ubiquitin ligase activity and promotes ubiquitylation. CHIP enhances HSP70 induction during acute stress and also mediates its turnover during the stress recovery process. The deduced 303-amino acid proteinhas a molecular mass of 35 kD and contains three 34-amino acid TPR domains at its N terminus, a central domain rich in charged residues, and 2 potential nuclear localization signals. Human CHIP shares 97% and 53% amino acid identity with its mouse and Drosophila homologs, respectively, with the highest conservation in the 94 residues of the C terminus.

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Mfr.No.	KTE60397
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human E3 ubiquitin-protein ligase CHIP (STUB1) ELISA Kit employs a two-site sandwich ELISA to quantitate STUB1.
Alternative Names	STUB1; LA16c-313D11.6; CHIP; HSPABP2; NY-CO-7; SDCCAG7; UBOX1; heat shock protein A binding protein 2 (c-terminal); serologically defined colon cancer antigen 7
Detection Method	Colorimetric
Kit Components	Human E3 ubiquitin-protein ligase CHIP microplate Human E3 ubiquitin-protein ligase CHIP standard Human E3 ubiquitin-protein ligase CHIP detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human E3 ubiquitin-protein ligase CHIP (STUB1) ELISA Kit has high sensitivity and excellent specificity for detection of Human STUB1. No significant cross-reactivity or interference between Human STUB1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	The CHIP protein encoded by this gene binds to and inhibits the ATPase activity of the chaperone proteins HSC70 and HSP70 and blocks the forward reaction of the HSC70-HSP70 substrate-binding cycle. In addition, CHIP possesses E3 ubiquitin ligase activity and promotes ubiquitylation. CHIP enhances HSP70 induction during acute stress and also mediates its turnover during the stress recovery process. The deduced 303-amino acid proteinhas a molecular mass of 35 kD and contains three 34-amino acid TPR domains at its N terminus, a central domain rich in charged residues, and 2 potential nuclear localization signals. Human CHIP shares 97% and 53% amino acid identity with its mouse and Drosophila homologs, respectively, with the highest conservation in the 94 residues of the C terminus.
Notes	This Human E3 ubiquitin-protein ligase CHIP (STUB1) ELISA Kit employs a two-site sandwich ELISA to quantitate STUB1 in samples. An antibody specific for STUB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySTUB1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for STUB1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of STUB1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	10273

Applications	ELISA
Applications Notes	This Human E3 ubiquitin-protein ligase CHIP (STUB1) ELISA Kit employs a two-site sandwich ELISA to quantitate STUB1 in samples. An antibody specific for STUB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySTUB1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for STUB1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of STUB1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702614	KTE60397	48 T	$339		Add to Cart
BHE13702614	KTE60397	96 T	$589		Add to Cart
BHE13702614	KTE60397	96 Tx5	$2629		Add to Cart
BHE13702614	KTE60397	96 Tx50	$24569		Add to Cart

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