Human dystroglycan 1 (dystrophin-associated glycoprotein 1) (DAG1) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-EL006488HU

Alternative Names

dystroglycan 1 (dystrophin-associated glycoprotein 1), 156DAG, A3a, AGRNR, DAG, alpha-dystroglycan, beta-dystroglycan, dystroglycan 1

Reactivity

Human

Detection Range

125 pg/mL-8000 pg/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of mouse NQO1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	86-98
EDTA plasma (n=4)	99	94-106

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse NQO1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	97
	Range %	92-104
1:2	Average %	94
	Range %	88-97
1:4	Average %	84
	Range %	80-88
1:8	Average %	89
	Range %	83-97

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Sensitivity

31.25 pg/mL

Limit Of Detection

450 nm

UniProt ID	Q14118
Antigen	dystroglycan 1 (dystrophin-associated glycoprotein 1)

Applications

ELISA

Research Area

DAG1

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

Have you used this product?

SKU	Supplier No.	Size	Your price
BHE10502176	CSB-EL006488HU	96T	$695	Add to Cart
BHE10502176	CSB-EL006488HU	5x96T	$3336	Add to Cart
BHE10502176	CSB-EL006488HU	10x96T	$6602.50	Add to Cart