Human Dynein Heavy Chain 1 - Axonemal

Mfr.No.

E4120hu

Description

This sandwich kit is for the accurate quantitative detection of human Dynein Heavy Chain 1,Axonemal (also known as DNAH1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Axonemal beta dynein heavy chain 1;Ciliary dynein heavy chain 1;DNAH 1;DNAH1;Dynein heavy chain 1, axonemal;hDHC7;Heat shock regulated protein 1;HSRF-1

Reactivity

Human

Detection Range

15-3000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

487478

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

8.21ng/L

Background

Force generating protein of cilia required for sperm flagellum motility. Produces force towards the minus ends of microtubules. Dynein has ATPase activity; the force-producing power stroke is thought to occur on release of ADP. Required in spermatozoa for the formation of the inner dynein arms and biogenesis of the axoneme (PubMed: 24360805).

Gene ID	25981
UniProt ID	Q9P2D7
Antigen	DNAH1

Research Area

Others

Protocol

These standard curves of E4120hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	1.945	1.951	1.894
	1.958
800ng/L	1.474	1.509	1.451
	1.544
400ng/L	0.548	0.612	0.554
	0.676
200ng/L	0.404	0.373	0.315
	0.341
100ng/L	0.145	0.174	0.116
	0.203
0ng/L	0.059	0.058	0
	0.057

Precision

We measured random samples of E4120hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	582.1	19.73	3.4
Intra-Assay	2	18	1138.5	78.83	6.9
Intra-Assay	3	18	385.8	14.15	3.7

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105525	E4120hu	48T	$320		Add to Cart
BHE12105525	E4120hu	96T	$458		Add to Cart

Human Dynein Heavy Chain 1 - Axonemal - DNAH1 ELISA Kit

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