Human Dualoxidase 1 - DUOX1 ELISA Kit

Mfr.No.

E5204Hu

Sensitivity

6.87ng/L

Detection Range

15-3000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Dual oxidase 1;DUOX1;Large NOX 1;LNOX1;Long NOX 1;NADPH thyroid oxidase 1;NOXEF1;THOX1;Thyroid oxidase 1;

Molecular Weight

177235

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E5204Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	740.33	26.96	3.64
Intra-Assay	2	18	506.48	24.58	4.85
Intra-Assay	3	18	212.45	9.76	4.59

Background

Generates hydrogen peroxide which is required for the activity of thyroid peroxidase/TPO and lactoperoxidase/LPO. Plays a role in thyroid hormones synthesis and lactoperoxidase-mediated antimicrobial defense at the surface of mucosa. May have its own peroxidase activity through its N-terminal peroxidase-like domain.

Protocol

These standard curves of E5204Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	2.113	2.144	2.11
	2.175
800ng/L	1.4	1.424	1.391
	1.449
400ng/L	0.742	0.733	0.699
	0.723
200ng/L	0.41	0.42	0.386
	0.429
100ng/L	0.175	0.151	0.118
	0.127
0ng/L	0.029	0.034	0
	0.038

Gene ID	53905
UniProt ID	Q9NRD9

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106567	E5204Hu	48T	$320		Add to Cart
BHE12106567	E5204Hu	96T	$458		Add to Cart

Human Dualoxidase 1 - DUOX1 ELISA Kit

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