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Home / ELISA Kit

Human Dual specificity protein phosphatase 6 (DUSP6) ELISA Kit

SKU : BHE13704181

DUSP6 is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily,which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates ERK2, is expressed in a variety of tissues with the highest levels in heart and pancreas, and unlike most other members of this family, is localized in the cytoplasm.

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Mfr.No.	KTE61967
Reactivity	Human
Sample Type	Serum, Cell culture supernatants, Plasma, Other biological fluids
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Dual specificity protein phosphatase 6 (DUSP6) ELISA Kit employs a two-site sandwich ELISA to quantitate DUSP6.
Alternative Names	DUSP6; MKP3; PYST1; MAP kinase phosphatase 3; serine/threonine specific protein phosphatase
Detection Method	Colorimetric
Kit Components	Human Dual specificity protein phosphatase 6 microplate Human Dual specificity protein phosphatase 6 standard Human Dual specificity protein phosphatase 6 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Dual specificity protein phosphatase 6 (DUSP6) ELISA Kit has high sensitivity and excellent specificity for detection of Human DUSP6. No significant cross-reactivity or interference between Human DUSP6 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	DUSP6 is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily,which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates ERK2, is expressed in a variety of tissues with the highest levels in heart and pancreas, and unlike most other members of this family, is localized in the cytoplasm.
Notes	This Human Dual specificity protein phosphatase 6 (DUSP6) ELISA Kit employs a two-site sandwich ELISA to quantitate DUSP6 in samples. An antibody specific for DUSP6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDUSP6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DUSP6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DUSP6 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	1848
Antigen	DUSP6

Applications	ELISA
Applications Notes	This Human Dual specificity protein phosphatase 6 (DUSP6) ELISA Kit employs a two-site sandwich ELISA to quantitate DUSP6 in samples. An antibody specific for DUSP6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDUSP6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DUSP6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DUSP6 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13704181	KTE61967	48 T	$339		Add to Cart
BHE13704181	KTE61967	96 T	$589		Add to Cart
BHE13704181	KTE61967	96 Tx5	$2629		Add to Cart
BHE13704181	KTE61967	96 Tx50	$24569		Add to Cart

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