Matrices listed below were spiked with certain level of dp-ucMGP and the recovery rates were calculated by comparing the measured value to the expected amount of dp-ucMGP in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-99
91
EDTA plasma(n=5)
85-101
93
heparin plasma(n=5)
85-103
93
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of dp-ucMGP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
92-104%
90-103%
87-102%
EDTA plasma(n=5)
89-95%
85-101%
88-98%
heparin plasma(n=5)
80-95%
81-88%
84-100%
Sensitivity
< 46.875pg/ml
Notes
For Research Use Only
UniProt ID
P08493
Applications
ELISA
Applications Notes
dp-ucMGPELISA Kit allows for the in vitro quantitative determination of dp-ucMGP concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human dp-ucMGP(Dephosphorylated Uncarboxylated Matrix Gla Protein)ELISA Kit