Human dopamine-beta-hydroxylase,DBH ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E09653h

Alternative Names

dopamine beta-hydroxylase (dopamine beta-monooxygenase), RP11-317B10.1, DBM, dopamine beta hydroxylase ,dopamine-beta-hydroxylase,DBH

Reactivity

Human

Detection Range

2.5 ng/mL-500 ng/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Competitive

Assay Duration

1-5h

Recovery

The recovery of rat F1+2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-98
EDTA plasma (n=4)	95	90-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat F1+2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	89
	Range %	80-92
1:2	Average %	100
	Range %	91-105
1:4	Average %	102
	Range %	92-110
1:8	Average %	93
	Range %	86-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Sensitivity

1.25 ng/mL

Limit Of Detection

450 nm

UniProt ID	P09172
Antigen	dopamine beta-hydroxylase (dopamine beta-monooxygenase)

Applications

ELISA

Research Area

DBH

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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