Human dopamine-beta-hydroxylase,DBH ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E09653h

Sensitivity

1.25 ng/mL

Detection Range

2.5 ng/mL-500 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Competitive

Sample Volume

50-100ul

Alternative Names

dopamine beta-hydroxylase (dopamine beta-monooxygenase), RP11-317B10.1, DBM, dopamine beta hydroxylase ,dopamine-beta-hydroxylase,DBH

Recovery

The recovery of rat F1+2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-98
EDTA plasma (n=4)	95	90-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat F1+2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	89
	Range %	80-92
1:2	Average %	100
	Range %	91-105
1:4	Average %	102
	Range %	92-110
1:8	Average %	93
	Range %	86-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Sample Type

Serum, Tissue homogenates, Plasma

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P09172

Applications	ELISA
Research Area	DBH

Storage	Store at 2-8°C. Please refer to protocol.

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