Human DNA Topoisomerase 2-beta

Mfr.No.

E5194Hu

Sensitivity

4.99ng/L

Detection Range

10-2000ng/L

Reactivity

Human

Sample Type

Serum, Cell culture supernatants, Plasma

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

DNA topoisomerase 2-beta;DNA topoisomerase II, beta isozyme;TOP 2B;TOP2B

Molecular Weight

183267

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E5194Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	370.6	17.34	4.7
Intra-Assay	2	18	592.1	19.27	3.3
Intra-Assay	3	18	199.3	13.43	6.7

Background

Key decatenating enzyme that alters DNA topology by binding to two double-stranded DNA molecules, generating a double-stranded break in one of the strands, passing the intact strand through the broken strand, and religating the broken strand.

Protocol

These standard curves of E5194Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200ng/L	2.536	2.585	2.534
	2.635
600ng/L	1.395	1.475	1.423
	1.555
300ng/L	0.815	0.796	0.745
	0.778
150ng/L	0.516	0.498	0.446
	0.479
75ng/L	0.205	0.204	0.152
	0.203
0ng/L	0.041	0.051	0
	0.062

Gene ID	7155
UniProt ID	Q02880
Antigen	TOP2B

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106557	E5194Hu	48T	$320		Add to Cart
BHE12106557	E5194Hu	96T	$458		Add to Cart

Human DNA Topoisomerase 2-beta - TOP2B ELISA Kit

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