Human DNA Repair Protein Xrcc1

Mfr.No.

E3435Hu

Sensitivity

12.5ng/L

Detection Range

25-1600ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

DNA repair protein XRCC1;RCC;SCAR26;X-ray repair cross-complementing protein 1;XRCC1

Molecular Weight

69477

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E3435Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	479.09	24.02	5.01
Intra-Assay	2	18	959.6	42.35	4.41
Intra-Assay	3	18	289.35	17.29	5.97

Background

Involved in DNA single-strand break repair by mediating the assembly of DNA break repair protein complexes. Probably during DNA repair, negatively regulates ADP-ribose levels by modulating ADP-ribosyltransferase PARP1 activity.

Protocol

These standard curves of E3435Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	2.188	2.154	2.091
	2.121
800ng/L	1.361	1.323	1.259
	1.284
400ng/L	0.801	0.846	0.783
	0.892
200ng/L	0.417	0.438	0.374
	0.459
100ng/L	0.234	0.242	0.179
	0.25
0ng/L	0.055	0.063	0
	0.072

Gene ID	7515
UniProt ID	P18887

Research Area	Epigenetics and Nuclear Signaling

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104957	E3435Hu	48T	$320		Add to Cart
BHE12104957	E3435Hu	96T	$458		Add to Cart

Human DNA Repair Protein Xrcc1 - XRCC1 ELISA Kit

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