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Home / ELISA Kit

Human DNA repair protein RAD51 homolog 1 (RAD51) ELISA Kit

SKU : BHE13703200

RAD51 is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Multiple transcript variants encoding different isoforms have been found for this gene.

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Mfr.No.	KTE60984
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human DNA repair protein RAD51 homolog 1 (RAD51) ELISA Kit employs a two-site sandwich ELISA to quantitate RAD51.
Alternative Names	RAD51; BRCC5; HRAD51; HsRad51; HsT16930; RAD51A; RECA; BRCA1/BRCA2-containing complex; subunit 5; DNA repair protein RAD51 homolog 1; RAD51 homolog protein; RecA; E. coli; homolog of; RecA-like protein; recombi
Detection Method	Colorimetric
Kit Components	Human DNA repair protein RAD51 homolog 1 microplate Human DNA repair protein RAD51 homolog 1 standard Human DNA repair protein RAD51 homolog 1 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human DNA repair protein RAD51 homolog 1 (RAD51) ELISA Kit has high sensitivity and excellent specificity for detection of Human RAD51. No significant cross-reactivity or interference between Human RAD51 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	RAD51 is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Multiple transcript variants encoding different isoforms have been found for this gene.
Notes	This Human DNA repair protein RAD51 homolog 1 (RAD51) ELISA Kit employs a two-site sandwich ELISA to quantitate RAD51 in samples. An antibody specific for RAD51 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRAD51 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RAD51 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RAD51 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	5888

Applications	ELISA
Applications Notes	This Human DNA repair protein RAD51 homolog 1 (RAD51) ELISA Kit employs a two-site sandwich ELISA to quantitate RAD51 in samples. An antibody specific for RAD51 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRAD51 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RAD51 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RAD51 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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