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Home / ELISA Kit

Human DNA primase small subunit (PRIM1) ELISA Kit

SKU : BHE13703374

The replication of DNA in eukaryotic cells is carried out by a complex chromosomal replication apparatus, in which DNA polymerase alpha and primase are two key enzymatic components. Primase, which is a heterodimer of a small subunit and a large subunit, synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication. The protein encoded by this gene is the small, 49 kDa primase subunit. The DNA polymerase-alpha/primase complex contains 4 subunits: the polymerase-alpha p180 (POLA) and p68 (POLA2) subunits, and the primase p58 (PRIM2A) and p49 (PRIM1) subunits. Primase synthesizes oligoribonucleotides that serve as primers for the initiation of DNA synthesis. It plays a role in both the initiation of DNA replication and the synthesis of Okazaki fragments for lagging strand synthesis

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Mfr.No.	KTE61158
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human DNA primase small subunit (PRIM1) ELISA Kit employs a two-site sandwich ELISA to quantitate PRIM1.
Alternative Names	PRIM1; MGC12308; p49; DNA primase 1; DNA primase polypeptide 1; DNA primase small subunit; DNA primase subunit 48; primase p49 subunit; primase polypeptide 1; 49kDa
Detection Method	Colorimetric
Kit Components	Human DNA primase small subunit microplate Human DNA primase small subunit standard Human DNA primase small subunit detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human DNA primase small subunit (PRIM1) ELISA Kit has high sensitivity and excellent specificity for detection of Human PRIM1. No significant cross-reactivity or interference between Human PRIM1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	The replication of DNA in eukaryotic cells is carried out by a complex chromosomal replication apparatus, in which DNA polymerase alpha and primase are two key enzymatic components. Primase, which is a heterodimer of a small subunit and a large subunit, synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication. The protein encoded by this gene is the small, 49 kDa primase subunit. The DNA polymerase-alpha/primase complex contains 4 subunits: the polymerase-alpha p180 (POLA) and p68 (POLA2) subunits, and the primase p58 (PRIM2A) and p49 (PRIM1) subunits. Primase synthesizes oligoribonucleotides that serve as primers for the initiation of DNA synthesis. It plays a role in both the initiation of DNA replication and the synthesis of Okazaki fragments for lagging strand synthesis
Notes	This Human DNA primase small subunit (PRIM1) ELISA Kit employs a two-site sandwich ELISA to quantitate PRIM1 in samples. An antibody specific for PRIM1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRIM1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRIM1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRIM1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	5557

Applications	ELISA
Applications Notes	This Human DNA primase small subunit (PRIM1) ELISA Kit employs a two-site sandwich ELISA to quantitate PRIM1 in samples. An antibody specific for PRIM1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRIM1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRIM1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRIM1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703374	KTE61158	48 T	$339		Add to Cart
BHE13703374	KTE61158	96 T	$589		Add to Cart
BHE13703374	KTE61158	96 Tx5	$2629		Add to Cart
BHE13703374	KTE61158	96 Tx50	$24569		Add to Cart

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