Human DNA-binding Protein Inhibitor Id-1

Mfr.No.

E5198Hu

Sensitivity

10.11ng/L

Detection Range

20-4500ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

bHLHb24;Class B basic helix-loop-helix protein 24;DNA-binding protein inhibitor ID-1;ID 1;ID1;Inhibitor of differentiation 1;Inhibitor of DNA binding 1

Molecular Weight

16133

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E5198Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	153	8.18	5.3
Intra-Assay	2	18	571.8	38.43	6.7
Intra-Assay	3	18	736.8	32.76	4.4

Background

Transcriptional regulator (lacking a basic DNA binding domain) which negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA binding and transcriptional activity. Implicated in regulating a variety of cellular processes, including cellular growth, senescence, differentiation, apoptosis, angiogenesis, and neoplastic transformation. Inhibits skeletal muscle and cardiac myocyte differentiation. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer (By similarity).

Protocol

These standard curves of E5198Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	2.137	2.159	2.125
	2.18
1200ng/L	1.884	1.813	1.778
	1.741
600ng/L	1.145	1.192	1.158
	1.238
300ng/L	0.664	0.639	0.605
	0.614
150ng/L	0.384	0.398	0.364
	0.412
0ng/L	0.041	0.034	0
	0.028

Gene ID	3397
UniProt ID	P41134

Research Area	Epigenetics and Nuclear Signaling

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106561	E5198Hu	48T	$320		Add to Cart
BHE12106561	E5198Hu	96T	$458		Add to Cart

Human DNA-binding Protein Inhibitor Id-1 - ID1 ELISA Kit

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