Human DNA-binding protein inhibitor ID-1(ID1) ELISA kit

Human DNA-binding protein inhibitor ID-1(ID1) ELISA kit

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Mfr.No.

CSB-EL010966HU

Sensitivity

7.81 pg/mL

Detection Range

31.25 pg/mL-2000 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

inhibitor of DNA binding 1, dominant negative helix-loop-helix protein, ID, bHLHb24, DNA-binding protein inhibitor ID-1, dJ857M17.1.2 (inhibitor of DNA binding 1, dominant negative helix-loop-helix protein), inhibitor of DNA binding 1, inhibitor of differentiation 1

Recovery

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human LUM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	88
	Range %	85-92
1:2	Average %	97
	Range %	91-105
1:4	Average %	100
	Range %	92-110
1:8	Average %	93
	Range %	86-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
40	2.858	2.889	2.874	2.743
20	2.361	2.381	2.371	2.240
10	1.752	1.852	1.802	1.671
5	1.134	1.233	1.184	1.053
2.5	0.653	0.695	0.674	0.543
1.25	0.431	0.423	0.427	0.296
0.625	0.296	0.321	0.309	0.178
0	0.133	0.129	0.131	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P41134

Applications	ELISA
Research Area	ID1

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10502149	CSB-EL010966HU	96T	$695	Add to Cart
BHE10502149	CSB-EL010966HU	5x96T	$3336	Add to Cart
BHE10502149	CSB-EL010966HU	10x96T	$6602.50	Add to Cart

The recovery of human LUM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type

Average % Recovery

Serum (n=5)