Human DNA-(apurinic or apyrimidinic site) lyase (APEX1) ELISA kit

Human DNA-(apurinic or apyrimidinic site) lyase (APEX1) ELISA kit

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Mfr.No.

CSB-EL001900HU

Sensitivity

3.9 pg/mL

Detection Range

15.6 pg/mL-1000 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

APEX nuclease (multifunctional DNA repair enzyme) 1, APE, APE1, APEN, APEX, APX, HAP1, REF1, AP endonuclease class I, AP lyase, APEX nuclease 1, DNA-(apurinic or apyrimidinic site) lyase, apurinic/apyrimidinic (abasic) endonuclease, apurinic/apyrimidinic e

Recovery

The recovery of mouse PLA2G12A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	86	83-89
EDTA plasma (n=4)	90	87-93

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse PLA2G12A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	84
	Range %	8-89
1:2	Average %	98
	Range %	94-103
1:4	Average %	85
	Range %	82-89
1:8	Average %	98
	Range %	94-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
4000	2.410	2.324	2.367	2.178
2000	1.610	1.684	1.647	1.458
1000	1.085	1.034	1.060	0.871
500	0.700	0.732	0.716	0.527
250	0.468	0.478	0.473	0.284
125	0.388	0.398	0.393	0.204
62.5	0.260	0.259	0.260	0.071
0	0.191	0.187	0.189

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P27695

Applications	ELISA
Research Area	APEX1

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10502146	CSB-EL001900HU	96T	$695	Add to Cart
BHE10502146	CSB-EL001900HU	5x96T	$3336	Add to Cart
BHE10502146	CSB-EL001900HU	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.