Matrices listed below were spiked with certain level of DMD and the recovery rates were calculated by comparing the measured value to the expected amount of DMD in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
98-103
100
EDTA plasma(n=5)
86-103
92
heparin plasma(n=5)
87-105
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of DMD and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
DMD ELISA Kit allows for the in vitro quantitative determination of DMD concentrations in serum, plasma, tissue homogenates and other biological fluids.