Human DKK-3 ELISA Kit PicoKine®

Mfr.No.

EK1323

Sensitivity

<10 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.118	0.254	0.347	0.545	0.909	1.248	1.765	2.165

Description

Human DKK-3 ELISA Kit PicoKine® (96 Tests). Quantitate Human DKK3 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Dickkopf-related protein 3;Dickkopf-3;Dkk-3;hDkk-3;DKK3;REIC;UNQ258/PRO295;

Molecular Weight

38390 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: A22-I350

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human DKK-3

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	308	1957	5464	336	2016	5345
Standard deviation	17.24	133.07	262.27	26.80	139.1	272.59
CV (%)	6.8%	6.8%	4.8%	7.5%	6.9%	5.1%

Protein Function

Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a teary complex with the transmembrane protein KREMEN that promotes intealization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero- posterior axial patteing, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease (By similarity). .

Background

Dickkopf-related protein 3 is a protein that in humans is encoded by the DKK3 gene. This gene encodes a protein that is a member of the dickkopf family. It is mapped to 11p15.3. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. The expression of this gene is decreased in a variety of cancer cell lines and it may function as a tumor suppressor gene. Members of the Dkk-related family display unique pattes of mRNA expression in human and mouse tissues, and are secreted when expressed in 293T cells. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patteing, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	308	334	342	343	331	14.14	4.2%
Sample 2	1957	1955	1972	1710	1898	109.02	5.7%
Sample 3	5464	5826	5731	5903	5731	165.75	2.8%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	27122
Gene Name	DKK3
UniProt ID	Q9UBP4
Protein Name	Dickkopf-related protein 3
Tissue Specificity	Highest expression in heart, brain, and spinal cord. .

Applications	ELISA
Research Area	Adapters,Secreted,Signal Transduction,Signaling Pathways,Stem Cells,Transmembrane

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000924	EK1323	96 wells/kit	$499		Add to Cart

Human DKK-3 ELISA Kit PicoKine®

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