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Home / ELISA Kit

Human Disintegrin and metalloproteinase domain-containing protein 28 (ADAM28) ELISA Kit

SKU : BHE13703130

ADAM28 encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene is a lymphocyte-expressed ADAM protein.
Expressed predominantly in secondary lymphoid tissues, such as lymph node, spleen, small intestine, stomach, colon, appendix and trachea. The lymphocyte population is responsible for expression of this protein in these tissues. Isoform 2 is expressed preferentially in spleen.

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Mfr.No.	KTE60914
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Disintegrin and metalloproteinase domain-containing protein 28 (ADAM28) ELISA Kit employs a two-site sandwich ELISA to quantitate ADAM28.
Alternative Names	ADAM28; ADAM23; MDC-Lm; MDC-Ls; MDCL; eMDCII; a disintegrin and metalloproteinase domain 28; metalloproteinase-like; disintegrin-like; and cysteine-rich protein-L
Detection Method	Colorimetric
Kit Components	Human Disintegrin and metalloproteinase domain-containing protein 28 microplate Human Disintegrin and metalloproteinase domain-containing protein 28 standard Human Disintegrin and metalloproteinase domain-containing protein 28 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Disintegrin and metalloproteinase domain-containing protein 28 (ADAM28) ELISA Kit has high sensitivity and excellent specificity for detection of Human ADAM28. No significant cross-reactivity or interference between Human ADAM28 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	ADAM28 encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene is a lymphocyte-expressed ADAM protein. Expressed predominantly in secondary lymphoid tissues, such as lymph node, spleen, small intestine, stomach, colon, appendix and trachea. The lymphocyte population is responsible for expression of this protein in these tissues. Isoform 2 is expressed preferentially in spleen.
Notes	This Human Disintegrin and metalloproteinase domain-containing protein 28 (ADAM28) ELISA Kit employs a two-site sandwich ELISA to quantitate ADAM28 in samples. An antibody specific for ADAM28 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyADAM28 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADAM28 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADAM28 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	10863

Applications	ELISA
Applications Notes	This Human Disintegrin and metalloproteinase domain-containing protein 28 (ADAM28) ELISA Kit employs a two-site sandwich ELISA to quantitate ADAM28 in samples. An antibody specific for ADAM28 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyADAM28 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADAM28 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADAM28 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703130	KTE60914	48 T	$339		Add to Cart
BHE13703130	KTE60914	96 T	$589		Add to Cart
BHE13703130	KTE60914	96 Tx5	$2629		Add to Cart
BHE13703130	KTE60914	96 Tx50	$24569		Add to Cart

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