Human dipeptidyl peptidase IV,DPPIV ELISA Kit

Human dipeptidyl peptidase IV,DPPIV ELISA Kit

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Mfr.No.

CSB-E08518h

Sensitivity

0.39 ng/mL

Detection Range

1.56 ng/mL-100 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

dipeptidyl-peptidase 4, ADABP, ADCP2, CD26, DPPIV, TP103, T-cell activation antigen CD26, adenosine deaminase complexing protein 2, dipeptidylpeptidase 4, dipeptidylpeptidase IV, dipeptidyl peptidase IV (CD26, adenosine deamina, dipeptidyl peptidase 4,DPP4,

Recovery

The recovery of human GALP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	92-102
EDTA plasma (n=4)	94	88-97

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human GALP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	87-97
1:2	Average %	101
	Range %	97-103
1:4	Average %	95
	Range %	88-98
1:8	Average %	99
	Range %	94-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.057	2.080	2.069	1.918
5	1.761	1.812	1.787	1.636
2.5	1.403	1.373	1.388	1.237
1.25	0.988	0.973	0.981	0.830
0.625	0.703	0.727	0.715	0.564
0.312	0.477	0.497	0.487	0.336
0.156	0.322	0.331	0.327	0.176
0	0.150	0.152	0.151

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P27487

Applications	ELISA
Research Area	DPP4

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10502130	CSB-E08518h	96T	$790	Add to Cart
BHE10502130	CSB-E08518h	5x96T	$3792	Add to Cart
BHE10502130	CSB-E08518h	10x96T	$7505	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.