Human Dipeptidyl Peptidase 3

Mfr.No.

E3112Hu

Sensitivity

2.21ng/ml

Detection Range

5-400ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Dipeptidyl Peptidase 3 (also known as DPP-3) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Dipeptidyl aminopeptidase III;Dipeptidyl arylamidase III;Dipeptidyl peptidase 3;Dipeptidyl peptidase III;DPP 3;DPP III;DPP3;Enkephalinase B

Molecular Weight

82589

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E3112Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	22.4	1.51	6.8
Intra-Assay	2	18	61.5	3.12	5.1
Intra-Assay	3	18	105.6	5.44	5.1

Background

Cleaves and degrades bioactive Peptides, including angiotensin, Leu-enkephalin and Met-enkephalin (PubMed: 3233187, PubMed: 1515063). Also cleaves Arg-Arg-beta-naphthylamide (in vitro) (PubMed: 9425109, PubMed: 3233187, PubMed: 11209758).

Protocol

These standard curves of E3112Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
240ng/ml	2.231	2.145	2.107
	2.058
120ng/ml	1.685	1.712	1.674
	1.738
60ng/ml	0.979	1.07	1.032
	1.161
30ng/ml	0.721	0.686	0.648
	0.651
15ng/ml	0.48	0.446	0.408
	0.413
0ng/ml	0.035	0.038	0
	0.041

Gene ID	10072
UniProt ID	Q9NY33

Research Area	Cell Biology

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104644	E3112Hu	48T	$320		Add to Cart
BHE12104644	E3112Hu	96T	$458		Add to Cart

Human Dipeptidyl Peptidase 3 - DPP3 ELISA Kit

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