Human Dipeptidase 1 - DPEP1 ELISA Kit

Mfr.No.

E5186Hu

Sensitivity

7.63ng/L

Detection Range

15-3000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Beta-lactamase;Dehydropeptidase-I;Dipeptidase 1;DPEP 1;DPEP1;hRDP;Microsomal dipeptidase;Renal dipeptidase

Molecular Weight

45674

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E5186Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	948	44.54	4.7
Intra-Assay	2	18	1419.9	38.79	2.7
Intra-Assay	3	18	544	27.03	5

Background

Hydrolyzes a wide range of dipeptides including the conversion of leukotriene D4 to leukotriene E4 (PubMed: 2303490, PubMed: 6334084, PubMed: 31442408). Hydrolyzes cystinyl-bis-glycine (cys-bis-gly) formed during glutathione degradation (By similarity). Possesses also beta lactamase activity and hydrolytically inactivates beta-lactam antibiotics (PubMed: 6334084). Independently of its dipeptidase activity acts as an adhesion receptor for neutrophils recruitement from bloodstream into inflammed lungs and liver.

Protocol

These standard curves of E5186Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	1.989	1.845	1.806
	1.701
800ng/L	0.915	0.899	0.86
	0.883
400ng/L	0.434	0.536	0.497
	0.637
200ng/L	0.219	0.245	0.206
	0.271
100ng/L	0.094	0.094	0.055
	0.094
0ng/L	0.026	0.039	0
	0.051

Gene ID	1800
UniProt ID	P16444

Research Area	Cancer

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106549	E5186Hu	48T	$320		Add to Cart
BHE12106549	E5186Hu	96T	$458		Add to Cart

Human Dipeptidase 1 - DPEP1 ELISA Kit

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