Matrices listed below were spiked with certain level of DIO3 and the recovery rates were calculated by comparing the measured value to the expected amount of DIO3 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
90-102
94
EDTA plasma(n=5)
94-104
99
heparin plasma(n=5)
93-102
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of DIO3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
DIO3/Type III iodothyronine deiodinase/Type-III 5'-deiodinase/Type 3 DI/5DIII/DIOIII
Notes
For Research Use Only
UniProt ID
P55073
Antigen
DIO3
Applications
ELISA
Applications Notes
DIO3 ELISA Kit allows for the in vitro quantitative determination of DIO3 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human DIO3(Type III iodothyronine deiodinase) ELISA Kit