Matrices listed below were spiked with certain level of DIO2 and the recovery rates were calculated by comparing the measured value to the expected amount of DIO2 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-102
96
EDTA plasma(n=5)
87-95
90
heparin plasma(n=5)
87-101
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of DIO2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
DIO2 ELISA Kit allows for the in vitro quantitative determination of DIO2 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human DIO2(Type II iodothyronine deiodinase) ELISA Kit