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Home / ELISA Kit

Human Dihydrotestosterone (DHT) ELISA Kit

SKU : BHE13704278

Dihydrotestosterone (DHT), or 5alpha-dihydrotestosterone (5alpha-DHT), also known as 5alpha-androstan-17beta-ol-3-one, is an endogenous androgen sex steroid and hormone. The enzyme 5alpha-reductase catalyzes the formation of DHT from testosterone in certain tissues including the prostate gland, seminal vesicles, epididymides, skin, hair follicles, liver, and brain. This enzyme mediates reduction of the C4-5 double bond of testosterone. Relative to testosterone, DHT is considerably more potent as an agonist of the androgen receptor (AR).

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Mfr.No.	KTE62064
Reactivity	Human
Sample Type	Serum, Cell culture supernatants, Plasma, Other biological fluids
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Dihydrotestosterone (DHT) ELISA Kit employs a two-site sandwich ELISA to quantitate DHT.
Alternative Names	DHT
Detection Method	Colorimetric
Kit Components	Human Dihydrotestosterone microplate Human Dihydrotestosterone standard Human Dihydrotestosterone detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Dihydrotestosterone (DHT) ELISA Kit has high sensitivity and excellent specificity for detection of Human DHT. No significant cross-reactivity or interference between Human DHT and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Dihydrotestosterone (DHT), or 5alpha-dihydrotestosterone (5alpha-DHT), also known as 5alpha-androstan-17beta-ol-3-one, is an endogenous androgen sex steroid and hormone. The enzyme 5alpha-reductase catalyzes the formation of DHT from testosterone in certain tissues including the prostate gland, seminal vesicles, epididymides, skin, hair follicles, liver, and brain. This enzyme mediates reduction of the C4-5 double bond of testosterone. Relative to testosterone, DHT is considerably more potent as an agonist of the androgen receptor (AR).
Notes	This Human Dihydrotestosterone (DHT) ELISA Kit employs a two-site sandwich ELISA to quantitate DHT in samples. An antibody specific for DHT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDHT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DHT is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DHT bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Antigen	DHT

Applications	ELISA
Applications Notes	This Human Dihydrotestosterone (DHT) ELISA Kit employs a two-site sandwich ELISA to quantitate DHT in samples. An antibody specific for DHT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDHT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DHT is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DHT bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13704278	KTE62064	48 T	$359		Add to Cart
BHE13704278	KTE62064	96 T	$619		Add to Cart
BHE13704278	KTE62064	96 Tx5	$2809		Add to Cart
BHE13704278	KTE62064	96 Tx50	$26209		Add to Cart

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