Human Dihydrotestosterone,DHT ELISA Kit

Human Dihydrotestosterone,DHT ELISA Kit

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Mfr.No.

CSB-E07878h

Alternative Names

Dihydrotestosterone,DHT

Reactivity

Human

Detection Range

10 pg/mL-2000 pg/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Competitive

Assay Duration

1-5h

Recovery

The recovery of human F13A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	84	80-88
EDTA plasma (n=4)	100	95-105

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human F13A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	85
	Range %	81-89
1:2000	Average %	93
	Range %	90-96
1:4000	Average %	87
	Range %	83-90
1:8000	Average %	100
	Range %	97-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
40	2.783	2.798	2.791	2.622
20	2.324	2.368	2.346	2.177
10	1.768	1.760	1.764	1.595
5	1.153	1.168	1.161	0.992
2.5	0.691	0.672	0.682	0.513
1.25	0.401	0.406	0.404	0.235
0.625	0.295	0.276	0.286	0.117
0	0.170	0.168	0.169

Species

Homo sapiens

Sensitivity

5 pg/mL

Limit Of Detection

450 nm

Antigen	Dihydrotestosterone,DHT

Applications

ELISA

Research Area

DHT

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

Reference	THE EFFECTS OF PHYLLANTHUS GOMPHOCARPUS HOOK. F. AQUEOUS EXTRACT SUPPLEMENTATION ON ANDROGENIC HORMONAL LEVEL AND HISTOLOGICAL TESTES MORPHOMETRY Ebby Anuar Bahari.et al,Jurnal Teknologi,78(6-7).

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.