Human Diamine acetyltransferase 1(SAT1) ELISA kit

Human Diamine acetyltransferase 1(SAT1) ELISA kit

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Mfr.No.

CSB-EL020717HU

Sensitivity

0.039 ng/mL

Detection Range

0.156 ng/mL-10 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

spermidine/spermine N1-acetyltransferase 1, DC21, KFSD, KFSDX, SAT, SSAT, SSAT-1, diamine N-acetyltransferase 1, diamine acetyltransferase 1, spermidine/spermine N1-acetyltransferase alpha

Recovery

The recovery of human IGF2BP3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	88-96
EDTA plasma (n=4)	97	93-102

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human IGF2BP3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-95
1:2	Average %	102
	Range %	97-107
1:4	Average %	91
	Range %	85-97
1:8	Average %	97
	Range %	91-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2400	2.784	2.799	2.792	2.640
1200	1.956	1.906	1.931	1.779
600	1.095	1.123	1.109	0.957
300	0.637	0.665	0.651	0.499
150	0.419	0.451	0.435	0.283
75	0.338	0.342	0.340	0.188
37.5	0.247	0.268	0.258	0.106
0	0.151	0.153	0.152

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P21673

Applications	ELISA
Research Area	SAT1

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10502116	CSB-EL020717HU	96T	$695	Add to Cart
BHE10502116	CSB-EL020717HU	5x96T	$3336	Add to Cart
BHE10502116	CSB-EL020717HU	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.