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Home / ELISA Kit

Human Diacylglycerol kinase alpha (DGKA) ELISA Kit

SKU : BHE13704297

The protein encoded by DGKA belongs to the eukaryotic diacylglycerol kinase family. It acts as a modulator that competes with protein kinase C for the second messenger diacylglycerol in intracellular signaling pathways. It also plays an important role in the resynthesis of phosphatidylinositols and phosphorylating diacylglycerol to phosphatidic acid. Several transcript variants encoding different isoforms have been identified for DGKA.

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Mfr.No.	KTE62083
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Diacylglycerol kinase alpha (DGKA) ELISA Kit employs a two-site sandwich ELISA to quantitate DGKA.
Alternative Names	DGKA; DAGK; DAGK1; DGK-alpha; MGC12821; MGC42356; diacylglycerol kinase alpha
Detection Method	Colorimetric
Kit Components	Human Diacylglycerol kinase alpha microplate Human Diacylglycerol kinase alpha standard Human Diacylglycerol kinase alpha detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Diacylglycerol kinase alpha (DGKA) ELISA Kit has high sensitivity and excellent specificity for detection of Human DGKA. No significant cross-reactivity or interference between Human DGKA and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	The protein encoded by DGKA belongs to the eukaryotic diacylglycerol kinase family. It acts as a modulator that competes with protein kinase C for the second messenger diacylglycerol in intracellular signaling pathways. It also plays an important role in the resynthesis of phosphatidylinositols and phosphorylating diacylglycerol to phosphatidic acid. Several transcript variants encoding different isoforms have been identified for DGKA.
Notes	This Human Diacylglycerol kinase alpha (DGKA) ELISA Kit employs a two-site sandwich ELISA to quantitate DGKA in samples. An antibody specific for DGKA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDGKA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DGKA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DGKA bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	1606

Applications	ELISA
Applications Notes	This Human Diacylglycerol kinase alpha (DGKA) ELISA Kit employs a two-site sandwich ELISA to quantitate DGKA in samples. An antibody specific for DGKA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDGKA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DGKA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DGKA bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13704297	KTE62083	48 T	$339		Add to Cart
BHE13704297	KTE62083	96 T	$589		Add to Cart
BHE13704297	KTE62083	96 Tx5	$2629		Add to Cart
BHE13704297	KTE62083	96 Tx50	$24569		Add to Cart

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