Human desmosine(DES) ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E12871h

Sensitivity

0.039 ng/mL

Detection Range

0.156 ng/mL-10 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

desmosine(DES)

Recovery

The recovery of pig IL-8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	95-104
EDTA plasma (n=4)	85	81-90

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	95
	Range %	91-99
1:2	Average %	90
	Range %	86-94
1:4	Average %	88
	Range %	84-93
1:8	Average %	92
	Range %	88-97

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	DES

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Procollagen III N-terminal propeptide and desmosine are released by matrix destruction in pulmonary tuberculosis Seddon J et al,J Infect Dis,2013 Aug 9. Induction and regulation of murine emphysema by elastin peptides Sellami M. et al,Am J Physiol Lung Cell Mol Physiol,2015 Oct 30

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