Human desmogleins 1,Dsg-1 ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E09592h

Alternative Names

desmoglein 1, CDHF4, DG1, DSG, PPKS1, SPPK1, desmosomal glycoprotein 1, pemphigus foliaceus antigen

Reactivity

Human

Detection Range

23.5 pg/mL-1500 pg/mL

Sample Type

Tissue homogenates, Cell lysates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human RCAS1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	92-102
EDTA plasma (n=4)	100	95-105

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human RCAS1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	94
	Range %	90-98
1:2	Average %	96
	Range %	91-101
1:4	Average %	100
	Range %	95-106
1:8	Average %	105
	Range %	99-111

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Sensitivity

5.8 pg/mL

Limit Of Detection

450 nm

UniProt ID	Q02413
Antigen	Desmoglein 1

Applications

ELISA

Research Area

DSG1

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

Have you used this product?

SKU	Supplier No.	Size	Your price
BHE10502111	CSB-E09592h	96T	$790	Add to Cart
BHE10502111	CSB-E09592h	5x96T	$3792	Add to Cart
BHE10502111	CSB-E09592h	10x96T	$7505	Add to Cart