Human Desmoglein-3(DSG3) ELISA kit

Mfr.No.

CSB-EL007205HU

Sensitivity

7 pg/mL

Detection Range

28 pg/mL-1800 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

desmoglein 3 (pemphigus vulgaris antigen), CDHF6, DKFZp686P23184, PVA, 130-kD pemphigus vulgaris antigen, desmoglein 3, pemphigus vulgaris antigen

Recovery

The recovery of human sTNF-R2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	100	94-104
EDTA plasma (n=4)	101	92-104

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human sTNF-R2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	101
	Range %	95-105
1:2	Average %	93
	Range %	87-97
1:4	Average %	97
	Range %	85-101
1:8	Average %	94
	Range %	85-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	P32926

Applications	ELISA
Research Area	DSG3

Storage	Store at 2-8°C. Please refer to protocol.

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