Human Delta Like Protein 4

Mfr.No.

E2466Hu

Sensitivity

7.82ng/L

Detection Range

15-3000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Delta like Protein 4 (also known as DLL4) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Delta4;Delta-like protein 4;DLL 4;DLL4;Drosophila Delta homolog 4

Molecular Weight

74605

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E2466Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	606.1	33.3	5.5
Intra-Assay	2	18	275	17.92	6.5
Intra-Assay	3	18	206.8	16.2	7.8

Background

Involved in the Notch signaling pathway as Notch ligand (PubMed: 11134954). Activates NOTCH1 and NOTCH4. Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting (PubMed: 20616313). Essential for retinal progenitor proliferation. Required for suppressing rod fates in late retinal progenitors as well as for proper generation of other retinal cell types (By similarity). During spinal cord neurogenesis, inhibits V2a interneuron fate (PubMed: 17728344).

Protocol

These standard curves of E2466Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	2.382	2.271	2.228
	2.16
800ng/L	1.688	1.701	1.658
	1.715
400ng/L	1.036	1.074	1.031
	1.113
200ng/L	0.664	0.668	0.625
	0.671
100ng/L	0.481	0.485	0.441
	0.488
0ng/L	0.032	0.043	0
	0.055

Gene ID	54567
UniProt ID	Q9NR61

Research Area	Developmental Biology

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104004	E2466Hu	48T	$320		Add to Cart
BHE12104004	E2466Hu	96T	$458		Add to Cart

Human Delta Like Protein 4 - DLL4 ELISA Kit

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