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Home / ELISA Kit

Human Delta (24)-sterol reductase (DHCR24) ELISA Kit

SKU : BHE13704288

DHCR24 encodes a flavin adenine dinucleotide (FAD)-dependent oxidoreductase which catalyzes the reduction of the delta-24 double bond of sterol intermediates during cholesterol biosynthesis. The protein contains a leader sequence that directs it to the endoplasmic reticulum membrane. Missense mutations in DHCR24 have been associated with desmosterolosis. Also, reduced expression of DHCR24 occurs in the temporal cortex of Alzheimer disease patients and overexpression has been observed in adrenal gland cancer cells.

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Mfr.No.	KTE62074
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Delta (24)-sterol reductase (DHCR24) ELISA Kit employs a two-site sandwich ELISA to quantitate DHCR24.
Alternative Names	DHCR24; KIAA0018; Nbla03646; SELADIN1; seladin-1; 3 beta-hydroxysterol delta 24-reductase; diminuto/dwarf1 homolog; putative protein product of Nbla03646; selective AD indicator 1
Detection Method	Colorimetric
Kit Components	Human Delta microplate Human Delta standard Human Delta detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Delta (24)-sterol reductase (DHCR24) ELISA Kit has high sensitivity and excellent specificity for detection of Human DHCR24. No significant cross-reactivity or interference between Human DHCR24 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	DHCR24 encodes a flavin adenine dinucleotide (FAD)-dependent oxidoreductase which catalyzes the reduction of the delta-24 double bond of sterol intermediates during cholesterol biosynthesis. The protein contains a leader sequence that directs it to the endoplasmic reticulum membrane. Missense mutations in DHCR24 have been associated with desmosterolosis. Also, reduced expression of DHCR24 occurs in the temporal cortex of Alzheimer disease patients and overexpression has been observed in adrenal gland cancer cells.
Notes	This Human Delta (24)-sterol reductase (DHCR24) ELISA Kit employs a two-site sandwich ELISA to quantitate DHCR24 in samples. An antibody specific for DHCR24 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDHCR24 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DHCR24 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DHCR24 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	1718

Applications	ELISA
Applications Notes	This Human Delta (24)-sterol reductase (DHCR24) ELISA Kit employs a two-site sandwich ELISA to quantitate DHCR24 in samples. An antibody specific for DHCR24 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDHCR24 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DHCR24 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DHCR24 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13704288	KTE62074	48 T	$339		Add to Cart
BHE13704288	KTE62074	96 T	$589		Add to Cart
BHE13704288	KTE62074	96 Tx5	$2629		Add to Cart
BHE13704288	KTE62074	96 Tx50	$24569		Add to Cart

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